Lady Macbeth’s eyes are open, but their sense is shut’; in common terms, she is sleepwalking. Lady Macbeth's sleep disorder reveals her inability to sleep; in addition, Lady Macbeth’s guilt and sinful deeds cause her to be restless. Furthermore, sleepwalking symbolizes distress and restlessness.
Darkness: Lady Macbeth says, “Hell is murky”; therefore, in a religious context, Lady Macbeth’s murderous acts reveal her disobedience to the “thou shalt not kill” commandment. Furthermore, the reference to hell symbolizes the concept and consequences of sin.
Blood
Lady Macbeth sleepwalks and imagines “a damned spot” (blood) on her hands. The blood represents her murderous acts towards King Duncan and Banquo. Therefore, the blood symbolizes her guilt. In addition, Lady Macbeth realizes her mistakes and attempts to rub the blood off of her hands; hence, she attempts to get rid of her guilt.
Rubbing Hands Lady Macbeth continuously rubs her hands together in an attempt to remove the imaginary blood. Lady Macbeth’s says, “Out, damned spot! Out, I say!” and "a little water clears us of the deed”, it shows her attempts to wash away her guilty conscience; therefore, washing her hands may represent the concept of cleansing her soul. In addition, it also symbolizes the concept of water as a purifier. In a religious context, this connects to baptism in water; therefore, washing her hands also symbolizes her salvation.
Light-Candle:
Lady Macbeth “has light by her continually”, it is her “command”. Firstly, Lady Macbeth’s obsession with always having a light in proximity symbolizes her fear of the dark; in addition, the dark also refers to Lady Macbeth's evil deeds. Secondly, Lady Macbeth realizes her mistakes; moreover, the light may also symbolize the concept of awareness.